Fig. 2

Fig. 2: Freshly isolated rat adipocytes [(4.5-5.5) x 106 cells/ml] were incubated with insulin or IP-oligosaccharides derived from Actovegin® hemodia-lysate for 20min at the concentrations given on the abscissa. The composition of the IP-oligosaccharide fraction was as follows: erythrose 2.54 mM (0.305 mg/ml), ribose 1.49 mM (0.166 mg/ml), arabinose 0.5 mM (0.150 mg/ml), xylose 1.26 mM (0.189 mg/ml), mannose 0.60 mM (0.108 mg/ ml), galactose 0.30 mM (0.053 mg/ml), glucose 1.22 mM (0.220 mg/ml), inositol 0.56 mM (0.100 mg/ml). The saccharides were complexed as phosphates or sulphates. The IP-oligosaccharide fraction is acid- and alkali-labile. 3-O-Methylglucose uptake was then determined for 4 s as described in the Materials and methods section and is expressed as % of equilibrium. The curves show mean values ±SEM of six experiments. Acetylation of the IP-oligosaccharide fraction decreased the effect of 27 or 270 ug/ml by approx. 50% (n — 2) Reprinted with permission from Ref. [9]

Fig. 2: Freshly isolated rat adipocytes [(4.5-5.5) x 106 cells/ml] were incubated with insulin or IP-oligosaccharides derived from Actovegin® hemodia-lysate for 20min at the concentrations given on the abscissa. The composition of the IP-oligosaccharide fraction was as follows: erythrose 2.54 mM (0.305 mg/ml), ribose 1.49 mM (0.166 mg/ml), arabinose 0.5 mM (0.150 mg/ml), xylose 1.26 mM (0.189 mg/ml), mannose 0.60 mM (0.108 mg/ ml), galactose 0.30 mM (0.053 mg/ml), glucose 1.22 mM (0.220 mg/ml), inositol 0.56 mM (0.100 mg/ml). The saccharides were complexed as phosphates or sulphates. The IP-oligosaccharide fraction is acid- and alkali-labile. 3-O-Methylglucose uptake was then determined for 4 s as described in the Materials and methods section and is expressed as % of equilibrium. The curves show mean values ±SEM of six experiments. Acetylation of the IP-oligosaccharide fraction decreased the effect of 27 or 270 ug/ml by approx. 50% (n — 2) Reprinted with permission from Ref. [9]

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